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Macrophages are phagocytic innate immune cells capable of phenotypical switching in response to the local microenvironment. Studies often use either primary macrophages or immortalized cell lines for hypothesis testing, therapeutic assessment, and biomaterial evaluation without carefully considering the potential effects of cell source and tissue of origin, which strongly influence macrophage response. Surprisingly, limited information is available about how, under similar stimuli, immortalized cell lines and primary cells respond in both phenotypical and functional changes. To address this need, in this work, we cultured immortalized macrophage cell lines derived from different origins (i.e.,blood, lung, peritoneal) to understand and compare macrophage phenotypical responses, including polarization and plasticity, morphological changes, and phagocytic functionalities, as well as compared primary macrophages extracted from peritoneal and bone marrow to their immortalized cell line counterparts. We found significant differences in baseline expression of different markers (e.g., CD86, MHCII, CD206, and EGR2) amongst different cell lines, which further influence both polarization and repolarization of the cells, in addition to their phagocytic functionality. Additionally, we observed that, while RAW 264.7 cells behave similarly to the primary bone marrow-derived macrophages, there are noticeable phenotypical and functional differences in cell line (IC-21) and primary peritoneal macrophages, highlighting tissue-specific differences in macrophage response amongst cell lines and primary cells. Moving to three-dimensional (3D) culture in well-defined biomaterials, blood-derived primary and cell line macrophages were encapsulated within hydrogel-based synthetic extracellular matrices and their polarization profiles and cell morphologies were compared. Macrophages exhibited less pronounced polarization during 3D culture in these compliant, soft materials compared to two-dimensional (2D) culture on rigid, tissue culture plastic plates. Overall, our findings highlight origin-specific differences in macrophage response, and therefore, careful considerations must be made to identify the appropriate cell source for the application of interest. 

This essay introduces an evidence-based teaching guide that presents research and resources on the uses and benefits of learning objectives (LOs). It summarizes key articles and includes a checklist, with the goal of encouraging instructors to incorporate LOs into their teaching practice. Ideas for further research are also discussed. 

There is much interest in integrating millimeter wave radios (mmWave) into wireless LANs and 5G cellular networks to benefit from their multi-GHz of available spectrum. Yet, unlike existing technologies, e.g., WiFi, mmWave radios require highly directional antennas. Since the antennas have pencil-beams, the transmitter and receiver need to align their beams before they can communicate. Existing systems scan the space to find the best alignment. Such a process has been shown to introduce up to seconds of delay and is unsuitable for wireless networks where an access point has to quickly switch between users and accommodate mobile clients. This paper presents Agile-Link, a new protocol that can find the best mmWave beam alignment without scanning the space. Given all possible directions for setting the antenna beam, Agile-Link provably finds the optimal direction in logarithmic number of measurements. Further, Agile-Link works within the existing 802.11ad standard for mmWave LAN, and can support both clients and access points. We have implemented Agile-Link in a mmWave radio and evaluated it empirically. Our results show that it reduces beam alignment delay by orders of magnitude. In particular, for highly directional mmWave devices operating under 802.11ad, the delay drops from over a second to 2.5 ms.